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Metabolism of the unnatural anticancer lipid safingol, L-threo-dihydrosphingosine, in cultured cells

By Mihaela Dragusin, Cristian Gurgui, Günter Schwarzmann and Joerg Hoernschemeyer


Abstract We studied the metabolism of radioactively labeled safingol (L-threo-dihydrosphingosine) in primary cultured neurons, B104 neuroblastoma cells, and Swiss 3T3 fibroblasts, and compared it to that of its natural stereoisomer D-erythro-dihydrosphingosine. Both sphingoid bases are used as biosynthetic precursors for complex sphingolipids, albeit to different rates. Whereas a considerable amount of the natural sphingoid base is also directed to the catabolic pathway (20–66%, cell type dependent), only a minor amount of the nonnatural safingol is subjected to catabolic cleavage, most of it being N-acylated to the respective stereochemical variant of dihydroceramide. Interestingly, N-acylation of safingol to L-threo-dihydroceramide is less sensitive to fumonisin B1 than the formation of the natural D-erythro-dihydroceramide. In addition, safingol-derived L-threo-dihydroceramide

Year: 2003
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