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Modulation of Glomerular Endothelin and Endothelin Receptor Gene Expression in Aminonucleoside-Induced

By Tsukasa Nakamura, Isao Ebihara, Mitsumine Fukui, Shiori Osada, Yasuhiko Tomino, Tomoh Masaki, Katsutoshi Goto, Yasuhiro Furuichi, Hikaru Koide, T. Nakamura, I. Ebihara, M. Fukul, S. Osada, V. Tomino and H. Koide


This study assessed glomerular endothelin (ET)-1, ET-3, and ET-receptor A and B mRNA levels in puromycin aminonucleoside (PAN)-induced nephrosis. During the nephrotic stage, 8 days after PAN injection, ET-1 and ETB receptor mRNA were elevated by 2.8 ± 0.8-fold (P < 0.01) and 2.4 ± 0.9-fold (P < 0.01), respectively, as compared with controls. These mRNA levels decreased to control levels by Day 20, when the nephrosis was In remission. In contrast, glomerular ETA receptor mRNA levels did not change in PAN nephrosis or control rats during the experimental penod. ET-3 mRNA was not detected in the glomeruli of PAN nephrosis or control rats. Additionally, plasma El concentration and glomerular El production were measured in PAN nephrosis and control rats by radioimmunoassay. Eight days after PAN injection, ET-1 levels in plasma and glomeruli were not significantly altered in rats with PAN-induced nephrosis (glomeruli, 104.68 ± 16.46 pg/mg of protein versus 98.24 ± 13.68 pg/mg of protein; plasma, 2.68 ± 1.10 versus 2.52 ± 0.98 pg/mL). The administration of methylprednisolone to PAN rats resulted in the rapid disappearance of proteinuria and partially attenuated the increased El-i and ETB receptor gene expression in the glomerull. These data indicate that glomerular ET-

Year: 2013
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