Location of Repository

Windows to cell function and dysfunction: signatures written in the boundary layers

By Peter J.S. Smith, Leon P. Collis and Mark A. Messerli

Abstract

The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments the profiles of which reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily on cultured cells but we extend our consideration to the far more complex environment of tissues, and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand

Topics: QD, QH301
Year: 2010
OAI identifier: oai:eprints.soton.ac.uk:188743
Provided by: e-Prints Soton

Suggested articles

Preview

Citations

  1. (2001). 5-hydroxytryptamine stimulates net Ca2+ flux in the ventricular muscle of a mollusc (Busycon canaliculatum) during cardioexcitation. doi
  2. A brief account of microscopical observations made in the months of June, July and August, 1827, on the particles contained in the pollen of plants; and on the general existence of active molecules in organic and inorganic bodies. doi
  3. (1913). A Contribution to the Theory of Electrocapillarity. doi
  4. (2004). A dialogue between glia and neurons in the retina: modulation of neuronal excitability. doi
  5. (2008). A novel three-phase model of brain tissue microstructure. doi
  6. (2006). A purinergic dialogue between glia and neurons in the retina. Novartis Found Symp. doi
  7. (1952). A quantitative description of membrane current and its application to conduction and excitation in nerve. doi
  8. (2006). Acid-mediated tumor invasion: a multidisciplinary study. Cancer Res.
  9. (1996). Actin microfilament disrupters enhance K(ATP) channel opening in patches from gui-nea-pig cardiomyocytes. doi
  10. (2001). Alkalinization by chloride/ bicarbonate pathway in larval mosquito midgut. doi
  11. (2002). Apoptosis recruits two-pore domain potassium channels used for homeostatic volume regulation. doi
  12. (2009). Astrocytic modulation of sleep homeostasis and cognitive consequences of sleep loss. doi
  13. (2005). Axonal excitability revisited. doi
  14. BCL-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. doi
  15. (2001). Ca 2+ signalling between single L-type C a2+ channels and ryanodine receptors in heart cells.
  16. (2001). Ca2+ signalling between single L-type Ca2+ channels and ryanodine receptors in heart cells.
  17. (2007). Characterization and inhibition of the recently discovered carbonic anhydrase isoforms CA XIII, XIV and XV. Curr Top Med Chem. doi
  18. (2008). Characterization of Optimized Na+ and Cl- liquid membranes for use with extracellular, self-referencing microelectrodes. doi
  19. (1910). Constitution of the Electric Charge at the Surface of an Electrolyte. doi
  20. (2000). Correlated oscillations in glucose consumption, oxygen consumption, and intracellular free Ca(2+) in single islets of Langerhans. doi
  21. (2001). Detection of Cl- flux in the apical microenvironment of cultured foetal distal lung epithelial cells.
  22. (1990). Detection of extracellular calcium gradients with a calcium specific vibrating electrode. doi
  23. (1999). Detection of local ATP release from activated platelets using cell surface-attached firefly luciferase.
  24. (2008). Dielectrophoretic assembly of insu- 50 linoma cells and fluorescent nanosensors into three-dimensional 'pseudo-islet' constructs. doi
  25. (2008). Dielectrophoretic assembly of insu-50 linoma cells and fluorescent nanosensors into three-dimensional 'pseudo-islet' constructs. doi
  26. (2000). Direct observation of calcium-independent intercellular ATP 10 signal-ing in astrocytes. doi
  27. (1966). Effect of nerve impulses on the membrane potential of glial cells in the central nervous system of amphibia.
  28. (1984). Effects of unstirred layers on membrane phenomena. Physiol Rev.
  29. (2006). Electrochemical sensor applications to the study of molecular physiology and analyte flux in plants. In: doi
  30. (2005). Electrokinetic measurements of membrane capacitance and conductance for pancreatic beta-cells. doi
  31. (1981). Electrostatic potential of bilayer lipid membranes with the structural surface charge smeared perpendicular to the membrane-solution interface. An extension of the Gouy-Chapman diffuse double layer theory. doi
  32. (2008). Extracellular acidification elicits spatially and temporally distinct Ca2+ signals. doi
  33. (2006). Fabrication and use of high-speed, concentric h+- and 30 Ca 2+-selective microelectrodes suitable for in vitro extracellular recording. doi
  34. (2006). Fabrication and use of high-speed, concentric h+- and 30 Ca2+-selective microelectrodes suitable for in vitro extracellular recording. doi
  35. Fine A 2003. Extracellular Ca 2+ depletion contributes to fast activity-dependent modulation of synaptic transmission in the brain. doi
  36. Fine A 2003. Extracellular Ca2+ depletion contributes to fast activity-dependent modulation of synaptic transmission in the brain. doi
  37. (1998). Geometric and viscous components of the tortuosity of the EICS in the brain. doi
  38. (2003). Glial cell inhibition of neurons by release of ATP.
  39. (1998). Impact of extracellular and intracellular diffusion on hepatic uptake kinetics. In: Whole Organ Approaches to Cellular Metabolism. doi
  40. (2008). Impact of microdialysis probes on vasculature and dopamine in the rat striatum: a combined fluorescence and voltammetric study. doi
  41. (2001). In situ analysis of pH gradients in mosquito larvae using non-invasive, self-referencing, pH-sensitive microelectrodes.
  42. (2006). In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain EICS. doi
  43. (1996). In vivo recording of calcium fluxes accompanying 'catch' contraction of molluscan smooth muscle.
  44. (2008). In-vivo electrochemistry: what can we learn about living systems? Chem Rev. doi
  45. (2008). Increased level of extracellular ATP at tumor sites: in vivo imaging with plasma membrane luciferase. doi
  46. (1997). Induced membrane hypo/hyper-mechanosensitivity: a limitation of 50 patch-clamp recording. doi
  47. (2003). Invasive consequences of using micro-electrodes and microdialysis probes in the brain. doi
  48. (1997). Invertebrate respiratory systems. doi
  49. (2003). Ion fluxes in giant excised cardiac membrane patches 20 detected and quantified with ion-selective microelectrodes. doi
  50. (2002). Ion regulation in the brain: implications for pathophysiology. doi
  51. (2008). Ion Selective and Amperometric Microelectrodes. P-97 Pipette Cookbook (pdf). Sutter Instruments.
  52. (2009). Ion trapping with fast response, ion-selective microelectrodes enhances detection of extracellular ion channel gradients . doi
  53. (1986). Ion-selective microelectrodes doi
  54. (2003). Left/right, up/down: the role of endogenous electrical fields as directional signals in development, repair and invasion. doi
  55. (2007). MAPK-dependent actin cytoskeletal reorganization underlies BK channel activation by insulin. doi
  56. (2001). Measurement of chloride flux associated with the myogenic response in rat cerebral arteries. doi
  57. (2007). Measuring extracellular ion gradients from single channels with ion-selective microelectrodes. doi
  58. (1998). Membrane surface- 50 charge titration probed by gramicidin A channel conductance. doi
  59. (1991). Membrane Technology and Dynamic Response of Ion-Selective Liquid-Membrane Electrodes. doi
  60. (2000). Metabolic oscillations in beta-cells.
  61. (1998). Microglia generate external proton and potassium ion gradients utilizing a member of the H/K ATPase family. doi
  62. (2006). Modulation of synaptic activity in Purkinje neurons by ATP. doi
  63. (2005). Monitoring Clmovement in single cells exposed to hypotonic solution. doi
  64. (2004). Multiple transport modes of the cardiac Na+/Ca2+ exchanger. doi
  65. (2004). Nanoscale probes encapsulated by biologically localized embedding (PEBBLEs) for ion sensing and imaging in live cells. doi
  66. (2004). Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. doi
  67. (2007). Neuronal somatic ATP release triggers neuronsatellite glial cell communication in dorsal root ganglia. doi
  68. (2004). Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate. doi
  69. (2007). New insights into the regulation of V-ATPase dependent proton secretion. doi
  70. (1994). Noninvasive measurement of interstitial pH profiles in normal and neoplastic tissue using fluorescence ratio imaging microscopy.Cancer Res.
  71. On Liquid Diffusion. doi
  72. On the Conductivity of Electrolytes Dissolved in Water in Relation to the Migration of their Components.
  73. (2001). Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography. doi
  74. (2007). Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs. doi
  75. (2007). pH dependence of melanoma cell migration: protons extruded by NHE1 dominate protons of the bulk solution. doi
  76. (2007). pH nanoenvironment at the surface of single melanoma cells. Cell Physiol Biochem. doi
  77. (1997). Physiological electrical fields modify cell behaviour. doi
  78. (1998). Plasma membrane Ca 2+-ATPase extrudes Ca 2+ from hair cell stereocilia.
  79. (2004). Potassium buffering in the central nervous system.Neuroscience. doi
  80. (1994). Potassium secretion by vestibular dark cell epithelium demonstrated by vibrating probe. doi
  81. (2007). Principles, development and applications of selfreferencing electrochemical microelectrodes to the determination of fluxes at cell membranes. In: Methods and New Frontiers in
  82. (2009). Protons make tumor cells move like clockwork. Pflugers Arch. doi
  83. (2008). Quantitative visualization of passive transport across bilayer lipid membranes Proc Natl Acad Sci USA.
  84. (2003). Regulation and modulation of pH in the brain. doi
  85. (2004). Regulation of neuronal ion channels via P2Y receptors. doi
  86. (2007). Regulation of postsynaptic Ca 2+ influx in hip- 40 pocampal CA1 pyramidal neurons via extracellular carbonic anhydrase. doi
  87. (2007). Regulation of postsynaptic Ca2+ influx in hip-40 pocampal CA1 pyramidal neurons via extracellular carbonic anhydrase. doi
  88. (1978). Response time studies on neutral carrier ion-selective membrane electrodes. doi
  89. (2009). Simultaneous Single Neuron Recording of O2 Consumption, [Ca2+]i and Mitochondrial Membrane Potential in Glutamate Toxicity. doi
  90. (1985). Sodium-pump density of cells from dog tracheal mucosa.
  91. (1975). Solutions of the Hodgkin-Huxley equations modified for potassium accumulation in a periaxonal space. Fed Proc. doi
  92. (1987). Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons. Nature 325:714-717. Draft for BioEssays 02/01/10PJSS doi
  93. (2003). Structural proton diffusion along lipid bilayers. doi
  94. (2007). Targeting vacuolar H+-ATPases as a new strategy against cancer. Cancer Res. doi
  95. (1956). The after-effects of impulses in the giant nerve fibres of Loligo. doi
  96. The phase-boundary potential model. doi
  97. (2009). The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and ex-changes 2 H + for each Ca 2+ or Ba 2+ ion extruded. doi
  98. (2009). The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and ex-changes 2 H+ for each Ca2+ or Ba2+ ion extruded. doi
  99. (2007). The spark of life: the role of electric fields in regulating cell behaviour using the eye as a model system. doi
  100. (2008). Transepithelial projections from basal cells are luminal sensors in pseudostratified epithelia. doi
  101. (1999). Transmembrane regulation of intracellular calcium by a plasma membrane sodium/calcium exchanger in mouse ova. doi
  102. (1998). Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. doi
  103. (1992). Working mechanism of ion-selective electrodes. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.