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Imaging the electric field associated with mouse and human skin wounds

By Richard Nuccitelli, Pamela Nuccitelli, Samdeo Ramlatchan, Richard Sanger and Peter J.S. Smith

Abstract

We have developed a noninvasive instrument called the bioelectric field imager (BFI) for mapping the electric field between the epidermis and the stratum corneum near wounds in both mouse and human skin. Rather than touching the skin, the BFI vibrates a small metal probe with a displacement of 180 ?m in air above the skin to detect the surface potential of the epidermis through capacitative coupling. Here we describe our first application of the BFI measuring the electric field between the stratum corneum and epidermis at the margin of skin wounds in mice. We measured an electric field of 177±14 (61) mV/mm immediately upon wounding and the field lines pointed away from the wound in all directions around it. Because the wound current flows immediately upon wounding, this is the first signal indicating skin damage. This electric field is generated at the outer surface of the epidermis by the outward flow of the current of injury. An equal and opposite current must flow within the multilayered epidermis to generate an intraepidermal field with the negative pole at the wound site. Because the current flowing within the multilayered epidermis is spread over a larger area, the current density and subsequent E field generated in that region is expected to be smaller than that measured by the BFI beneath the stratum corneum. The field beneath the stratum corneum typically remained in the 150–200 mV/mm range for 3 days and then began to decline over the next few days, falling to zero once wound healing was complete. The mean wound field strength decreased by 64±7% following the application of the sodium channel blocker, amiloride, to the skin near the wound and increased by 82±21% following the application of the Cl? channel activator, prostaglandin E2

Topics: QP, TK
Year: 2008
OAI identifier: oai:eprints.soton.ac.uk:188775
Provided by: e-Prints Soton

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  1. (1932). A new method of measuring contact potential differences in metals. Rev Sci Instr doi
  2. (2003). A role for endogenous electric fields in wound healing. Curr Top Dev Biol doi
  3. (1974). An ultrasensitive vibrating probe for measuring extracellular currents. doi
  4. (1974). An ultrasensitive vibrating probe for measuring extracellular currents. JCell Biol doi
  5. (2007). Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo. Nat Protoc doi
  6. (2007). Beta-adrenergic receptor agonists delay while antagonists accelerate epithelial wound healing: evidence of an endogenous adrenergic network within the corneal epithelium. doi
  7. (2002). Calcium channel blockers inhibit galvanotaxis in human keratinocytes. doi
  8. Contact electricity of metals. doi
  9. (2005). Controlling cell behavior electrically: current views and future potential. Physiol Rev
  10. Cyclic AMP mediates keratinocyte directional migration in an electric field. doi
  11. (2001). Cyclic AMP-dependent protein kinase A plays a role in the directed migration of human keratinocytes in a DC electric field. Cell Motil Cytoskeleton doi
  12. (2004). DC electric fields induce distinct preangiogenic responses in microvascular and macrovascular cells. Arterioscler Thromb Vasc Biol doi
  13. EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells. doi
  14. (2002). Electrical cues regulate the orientation and frequency of cell division and the rate of wound healing in vivo. doi
  15. (1986). Electrical fields in Notophthalmus viridescens limb stumps. Dev Biol doi
  16. (2006). Electrical signals control wound healing through phosphatidylinositol-3-OH kinase-gamma and PTEN. Nature doi
  17. (2008). Electrical stimulation for wound healing: a review of evidence from in vitro studies, animal experiments, and clinical trials. Int J Low Extrem Wounds 2005; 4: 23–44. Wound Rep Reg doi
  18. (2008). Electrophoresis along cell membranes. Nature (London) 1977; 265: 600–2. Wound Rep Reg doi
  19. (1998). Endogenous lateral electric fields around bovine corneal lesions are necessary for and can enhance normal rates of wound healing. Wound Repair Regen doi
  20. (1999). Epidermal growth factor receptor relocalization and kinase activity are necessary for directional migration of keratinocytes in DC electric fields.
  21. (1996). Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds.
  22. (1983). Human skin battery potentials and their possible role in wound healing. doi
  23. (1996). Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules. doi
  24. (2007). In vivo estimation of stratum corneum thickness from water concentration profiles obtained with Raman spectroscopy. Acta Derm Venereol doi
  25. (2006). Isseroff RR. beta4 integrin and epidermal growth factor coordinately regulate electric field mediated directional migration via Rac1. Mol Biol Cell doi
  26. (1998). Low cost PC based scanning Kelvin probe. Rev Sci Instr doi
  27. (1980). Measurement of electrical currents emerging during the regeneration of amputated fingertips in children. Clin Phys Physiol Meas doi
  28. (2002). Membrane lipids, EGF receptors, and intracellular signals colocalize and are polarized in epithelial cells moving directionally in a physiological electric field. doi
  29. (1998). Migration of human keratinocytes in electric fields requires growth factors and extracellular calcium. doi
  30. (1999). Multitip scanning bio-Kelvin probe. Rev Sci Instr doi
  31. (2007). Non-invasive measurement of bioelectric currents with a vibrating probe. Nat Protoc doi
  32. (1996). Orientation and directed migration of cultured corneal epithelial cells in small electric fields are serum dependent. doi
  33. (1988). Physiological electric fields can influence cell motility, growth, and polarity. doi
  34. (1993). Protein kinases are required for embryonic neural crest cell galvanotaxis. Cell Motil Cytoskeleton doi
  35. (1993). Protein kinases are required for embryonic neural crest cell galvanotaxis.Cell Motil Cytoskeleton doi
  36. (2000). The galvanotaxis response mechanism of keratinocytes can be modeled as a proportional controller. Cell Biochem Biophys doi
  37. (1982). The glabrous epidermis of cavies contains a powerful battery.Am J Physiol
  38. (1982). The glabrous epidermis ofcavies contains a powerful battery. Am JPhysiol
  39. (1976). Ultrastructural site variations in mouse epidermal organization.
  40. (1950). Vibrating probe electrometer for the measurement of bioelectric potentials. Rev Sci Instr doi
  41. Vorlaufiger abrifs einer untersuchung uber den sogenannten froschstrom und die electomotorischen fische. doi
  42. (2005). Wound healing in rat cornea: the role of electric currents. doi

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