In vivo expression technology was used for testing Pseudomonas aeruginosa in the rat lung model of chronic infection and in a mouse model of systemic infection. Three of the eight ivi proteins found showed sequence identity to known virulence factors involved in iron acquisition via an open reading frame (called pvdI) implicated in pyoverdine biosynthesis, membrane biogenesis (FtsY), and adhesion (Hag2). Pseudomonas aeruginosa is an opportunistic pathogen important in cystic fibrosis patients, for whom chronic P. aeruginosa infections remain the major cause of acute pneumonia, leading to debilitating lung malfunction and premature death. Although several P. aeruginosa virulence factors have been extensively studied in vitro, less is known about virulence factors during infection. Several approaches have been reported to allow the recovery, identification, and characterization of genes that are expressed in the host (2–4). We have utilized the in vivo expression technology (IVET) purA promoter trap system (5) to identify P. aeruginosa genes that are specificall
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