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Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection : a fast new approach to selective derivatization techniques

By Sercan (S30013) Pravadali-Cekic, Andrew (S32148) Jones, Artaches A. Kazarian, Brett Paull, Arianne Soliven, Harald J. Ritchie, Michelle Camenzuli, Lisa Leung, Gary R. (R7569) Dennis and Ross A. (R8557) Shalliker

Abstract

Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously

Topics: XXXXXX - Unknown, amino acids, chromatography, fluorescence, chromatography columns, derivatization
Publisher: U.S., Taylor and Francis
Year: 2017
DOI identifier: 10.1080/10826076.2017.1285312
OAI identifier: oai:nuws:uws_39195
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