Article thumbnail

A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

By Sonja Hess


The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields. To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents such as Marfey’s reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers

Publisher: 'Springer Science and Business Media LLC'
Year: 2019
OAI identifier:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.