Brief Definitive Report HIGHLY PURIFIED MURINE INTERLEUKIN 5 (IL-5) STIMULATES EOSINOPHIL FUNCTION AND PROLONGS IN VITRO SURVIVAL IL-5 as an Eosinophil Chemotactic Factor

Abstract

A B cell-active factor termed T cell-replacing factor (TRF) derived from the B151 T cell hybridoma has been shown to promote IgM secretion by the BCL I B cell line and to induce hapten-specific IgG secretion in vitro by in vivo antigen-primed B cells (1-3). Recently, the gene structure of TRF has been determined, and TRF has been revealed to enhance the production of IgA by mouse B cells (4) and induce IL-2 receptors on B cells (5). It also acts as eosinophil differentiation factor (6). Because of these multiple activities, this TRF was proposed to be called interleukin 5 (IL-5). More recently, we demonstrated that highly purified IL-5 supported the terminal differentiation and proliferation of murine eosinophilic precursors (7). In this study, to elucidate whether IL-5 increases the functional properties of mature eosinophils, we examined its effects on the survival, the superoxide anion production, and the chemotactic activities of murine mature eosinophils. Materials and Methods Hemopoietic Factors. We used TRF as rIL-5, which was synthesized as described previousl

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