Bovine chromaffin granule membranes undergo Ca(2�)-regulated exocytosis in frog oocytes


ments were performed with a subcellular fraction from cultured chromaffin cells enriched with [3H]norepinephrine-containing chrornaffin granules. Because the release of [3H]norepinephrine was strongly correlated with the appearance of dopamine-ß-hydroxylase on the oocyte surface, it is likely that intact chromaffin granules and chrornaffin granule membranes undergo exocytosis in the oocyte. Thus, the secretory vesicle membrane without normal vesicle contents is competent to undergo the sequence of events leading to exocytosis. Furthermore, the interchangeability of mammalian and amphibian components suggests substantial biochemical conservation of the regulated exocytotic pathway during the evolutionary progression from amphibians to mammals. Abstract. We have devised a new method that permit

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