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Histamine Type I Receptor Occupancy Increases Endothelial Cytosolic Calcium, Reduces F-Actin, and Promotes Albumin Diffusion Across

By Cultured Endothelial Monolayers, Daniel Rotrosen and John I. Gallin


Abstract, Considerable evidence suggests that Ca 2+ modulates endothelial cell metabolic and morphologic responses to mediators of inflammation. We have used the fluorescent Ca 2+ indicator, quin2, to monitor endothelial cell cytosolic free Ca 2+, [Ca2÷]~, in cultured human umbilical vein endothelial cells. Histamine stimulated an increase in [Ca2+] ~ from a resting level of 111 + 4 nM (mean __ _ SEM, n = 10) to micromolar levels; maximal and half-maximal responses were elicited by 10-4 M and 5 × 10-6 M histamine, respectively. The rise in [Ca2+]i occurred with no detectable latency, attained peak values 15-30 s after addition of stimulus, and decayed to a sustained elevation of [Ca2+] ~ two- to threefold resting. Hj receptor specificity was demonstrated for the histamine-stimulated change

Year: 2013
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