We present a refined method and design for fabricating parylene neurocages for in vitro studies of live neural networks. Parylene neurocages are biocompatible and very robust, making them ideally suited for studying the synaptic connections netween individual neurons to gain insight into learning and memory. The neurocage fabrication process is significantly less complex than earlier versions. Previous neurocage designs achieved limited neuronal outgrowth; however, the long-term cell survival rate was 50%
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