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JCB: ARTICLE Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures

By Chris Allen, Sabrina Büttner, Anthony D. Aragon, Jason A. Thomas, Osorio Meirelles, Jason E. Jaetao, Don Benn, Stephanie W. Ruby, Marten Veenhuis, Frank Madeo and Margaret Werner-washburne


Quiescence is the most common and, arguably, most poorly understood cell cycle state. This is in part because pure populations of quiescent cells are typically difficult to isolate. We report the isolation and characterization of quiescent and nonquiescent cells from stationary-phase (SP) yeast cultures by densitygradient centrifugation. Quiescent cells are dense, unbudded daughter cells formed after glucose exhaustion. They synchronously reenter the mitotic cell cycle, suggesting that they are in a G0 state. Nonquiescent cells are less dense

Year: 2011
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