Location of Repository

Use of Human Lymphocytes for Radiation Risk Assessment

By T. Größer, P. Hessel, S. Ritter (gsi and E. Nasonova (jinr


Chromosome aberrations are regarded as the most sensitive indicator of radiation-induced genetic alterations. In particular the frequency of aberrations in peripheral blood lymphocytes (PBLs) is used to estimate the dose to which an individual has been exposed. Moreover, aberrations in PBLs are considered as a promosing tool in predictive assay research aiming to develop a test for individual normal tissue tolerance. According to the standard protocol cytogenetic damage is analysed in first generation metaphases collected at one sampling interval. In the case of PBLs, metaphases are routinely harvested 48h after initiation of the culture. Yet, there is increasing evidence that radiation-induced cell cycle delays affect the aberration yield. In particular after high LET exposure a drastic increase in chromosomal damage with sampling time has been observed, i.e. heavily damaged cell

Year: 2011
OAI identifier: oai:CiteSeerX.psu:
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://citeseerx.ist.psu.edu/v... (external link)
  • http://www.gsi.de/annrep2001/f... (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.