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Characterizing Viscoelastic Properties of Polyacrylamide Gels

By Jeremy Ryan Griffin, Ploypan Thongpradit, Todd F. Peterson and Zachary R Gautreau

Abstract

Despite its common use in cell culture studies, polyacrylamide gel\u27s mechanical properties remain unclear. To characterize this gel fully, atomic force microscopy, uniaxial testing, rheology, and ball indentation were used. The range of the elastic moduli was 1.9-7.5 kPa and 18.1-39.4 kPa, for 5%/0.025% and 8%/0.08% gel concentrations, respectively, and a Poisson\u27s ratio of 0.45 was found. Future research utilizing polyacrylamide as a cell culture substrate should indicate how the gel was characterized and acknowledge the uncertainty in stiffness values

Publisher: Digital WPI
Year: 2006
OAI identifier: oai:digitalcommons.wpi.edu:mqp-all-1563
Provided by: DigitalCommons@WPI

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