The ability of systems of molecular reactions to be simultaneously autocatalytic and sustained by some food source of simple molecules may have been an essential step in the origin of life. In this report we will continue the work by Hordijk and Steel (2004) and Mossel and Steel (2005) of investigating the probability of autocatalytic sets arising. We first describe an alternative to the RAF algorithm in Hordijk and Steel (2004) that determines whether a system of molecules, reactions and catalysations contains a subsystem that is autocatalytic and able to be sustained from a given subset of molecules. We use the algorithm to investigate random catalytic networks. In particular two new models, Template based model and Thermodynamic model, will be described and investigated both analytically and numerically−it will be investigated if the rate of catalyses needed for the existence of autocatalytic and selfsustainin
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