Attach experimental protocol(s) that involve the use of cryogenic liquid. POTENTIAL HAZARDS Most cryogenic liquids can be placed into one of three groups: o Inert gases do not burn or support combustion; e.g., nitrogen, helium, neon, argon, krypton o Flammable gases produce gas that can burn in air; e.g., hydrogen, methane, liquefied natural gas o Liquid oxygen vigorously promotes combustion in otherwise non-combustible materials, like carbon and stainless steels, cast iron, aluminum, zinc, and Teflon. It also reacts explosively with organic materials. Avoid direct contact and exposure to cryogenic liquids as they • Can cause severe burns and frostbite. • Can cause asphyxiation, fire, and explosion from the evaporation of cryogens. • Will cause irritation if sprayed directly in the eye. • Can cause noise from stainless transfer line when filling large dewars. Containers may rupture or explode if exposed to heat. ENGINEERING CONTROLS • Ensure local exhaust ventilation system is in place. This will prevent the buildup of flammable gases or the displacement of breathable air. • Do not place cylinders in a closet or other enclosed space where fresh air is not supplied to the area (i.e. “cold ” room or “warm ” room). • Insulate the transfer line to reduce the noise when filling large dewars
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