We use two micro datasets that collect harmonized data across countries to investigate the effects of regulation on new businesses. We are able to distinguish between two types of entrepreneurs: those who start a business to pursue a business opportunity and those who could not find better work. Irrespective of the measure of regulation we use, we always find a detrimental effect of regulation on new businesses. While women are overall less likely to start new businesses, in more regulated countries women are pulled into entrepreneurship but not to pursue a business opportunity, only because they could not find better work. Moreover, regulation dampens the effects of self-assessed business skills and social networks. In more regulated economies, those with better business skills and those who know other entrepreneurs are less likely to become entrepreneurs to purse a business opportunity. Tighter regulation also exacerbates fear of failure, further discouraging business start-up. All our estimates point to a negative effect of regulation.