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Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

By Arnaud Javelle, Blanca-Rosa Rodrı́guez-Pastrana, Christophe Jacob, Bernard Botton, Annick Brun, Bruno André, Anne-Marie Marini and Michel Chalot

Abstract

AbstractHeterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Δ cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium

Publisher: Federation of European Biochemical Societies. Published by Elsevier B.V.
Year: 2001
DOI identifier: 10.1016/S0014-5793(01)02802-2
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