i Now a days, large quantities of gene sequences of related species of plants, animals and microorganisms show complex patterns of similarity to one another and many molecular biologists are convinced that an understanding of sequence evolution is the first step towards understanding the evolution itself. In fact this is one of the most fascinating aspects of the study of evolution. Thus the comparison of gene sequences or biological sequence analysis is one of the processes used to understand sequence evolution. Just as the ancient Greeks used comparative anatomy to understand the human body and linguists used the Rosetta stone to decipher Egyptian hieroglyphs, today we can use comparative sequence analysis to understand genomes. There is variety of different tools available to perform sequence analysis. Various DNA sequences alignment tools have been developed. Various software packages of automated tools have been developed that had improved the efficiency of much biological research. Fast, economical, flexible, and extensible computing power i
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