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mRNA Expression Analysis of Tissue Sections and Single Cells

By James Eberwine, Janet Estee Kacharmina, Christine Andrews, Kevin Miyashiro, Tracy Mcintosh, Kevin Becker, Tanya Barrett, Dave Hinkle, Gersham Dent and Paolo Marciano


Thirty years of literature have shown that changes in mRNA abundance provide insight into cellular functioning. Indeed, mRNA abundance measurements have been used to determine how effective particular drugs are in eliciting cellular responses, in monitoring behavioral responses, and in several other areas of neurobiology. Although investigation of individual mRNAs was informative, there are many thousands of mRNAs expressed in individual cells at varying abundances, the combination of which gives rise to cellular functioning. The advent of high-density cDNA arrays has greatly facilitated the ability to simultaneously examine the abundances of multiple mRNAs (Gress et al., 1992; Lockhart et al., 1996). There are many areas warranting comment when discussing microarrays, only a few of which can be discussed in a minireview. One issue of importance is the relative merit in screenin

Year: 2009
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