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Mutational Analysis and Membrane Topology of ComP, a Quorum-Sensing Histidine Kinase of Bacillus subtilis Controlling Competence Development

By Flavia Piazza, Pablo Tortosa and David Dubnau


ComP is a sensor histidine kinase of Bacillus subtilis required for the signal transduction pathway that initiates the development of competence for genetic transformation. It is believed that ComP senses the presence of ComX, a modified extracellular peptide pheromone, and donates a phosphate to ComA, thereby activating this transcription factor for binding to the srfA promoter. In the present study, fusions to the Escherichia coli proteins PhoA and LacZ and analysis of its susceptibility to the protease kallikrein were used to probe the membrane topology of ComP. These data suggest that ComP contains six or eight membranespanning segments and two large extracytoplasmic loops in its N-terminal membrane-associated domain. Deletions were introduced involving the large extracellular loops to explore the role of the N-terminal domain of ComP in signal transduction. The absence of the second loop conferred a phenotype in which ComP was active in the absence of ComX. The implications of these data are discussed. Competence development in Bacillus subtilis is controlled by a complex signal transduction pathway that culminates with the activation of genes encoding the machinery for DNA binding and uptake (for reviews, see references 11, 12, and 16). The histidine-kinase ComP (64) and its cognate respons

Year: 1999
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