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Isolation, Structure, Synthesis, and Activity of a New Member of the Calcitonin Gene-related Peptide Family from Frog Skin and Molecular Cloning of Its Precursor*

By Aurelia Anne Seon, Thierry Nicolas Pierre, Virginie Redeker and Claire Lacombe


Calcitonin gene-related peptide has been extracted from the skin exudate of a single living specimen of the frog Phyllomedusa bicolor and purified to homogeneity by a two-step protocol. A total volume of 250 �l of exudate yielded 380 �g of purified peptide. Mass spectrometric analysis and gas phase sequencing of the purified peptide as well as chemical synthesis and cDNA analysis were consistent with the structure SCDTSTCATQRLAD-FLSRSGGIGSPDFVPTDVSANSF amide and the presence of a disulfide bridge linking Cys 2 and Cys 7. The skin peptide, named skin calcitonin gene-related peptide, differs significantly from all other members of the calcitonin gene-related peptide family of peptides at nine positions but binds with high affinity to calcitonin gene-related peptide receptors in the rat brain and act

Year: 2009
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