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Interphase nuclei of many mammalian cell types contain deep, dynamic, tubular membranebound invaginations of the nuclear envelope

By Mark Fricker, Michael Hollinshead, Nick White and David Vaux


Abstract. The nuclear envelope consists of a doublemembraned extension of the rough endoplasmic reticulum. In this report we describe long, dynamic tubular channels, derived from the nuclear envelope, that extend deep into the nucleoplasm. These channels show cell-type specific morphologies ranging from single short stubs to multiple, complex, branched structures. Some channels transect the nucleus entirely, opening at two separate points on the nuclear surface, while others terminate at or close to nucleoli. These channels are distinct from other topological features of the nuclear envelope, such as lobes or folds. The channel wall consists of two membranes continuous with the nuclear envelope, studded with features indistinguishable from nuclear pore complexes, and decorated on the nucleoplasmic surface with lamins. The In recent years, the view that the nucleoplasm is organized into a number of morphologically distinct and functionally significant domains has gained ground, supported by increasing evidence for subnuclear localization of processes such as replication (Banfalvi et al., 1989; Mills et al., 1989; Hozak and Cook, 1994; Hutchison et al., 1994), repair (Jackson et al., 1994a, b), transcription (Jackson et al., 1993; Wansink et al., 1993), and RNA splicing and processing (Huang and Spector, 1991; Xing and Lawrence, 1993; Xing et al., 1993). In some cases these localized functions, or proteins associated with them, can be correlated with specific subnuclear structures, identified either morphologically or immunocytochemically at th

Year: 1997
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