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Targeting of tail-anchored proteins to yeast mitochondria in vivo

By Billie Egan, Traude Beilharz, Rebecca George, Sandra Isenmann, Sabine Gratzer, Binks Wattenberg and Trevor Lithgow

Abstract

AbstractTail-anchored proteins are inserted into intracellular membranes via a C-terminal transmembrane domain. The topology of the protein is such that insertion must occur post-translationally, since the insertion sequence is not available for membrane insertion until after translation of the tail-anchored polypeptide is completed. Here, we show that the targeting information in one such tail-anchored protein, translocase in the outer mitochondrial membrane 22, is contained in a short region flanking the transmembrane domain. An equivalent region is sufficient to specify the localisation of Bcl2 and SNARE proteins to the secretory membranes. We discuss the targeting process for directing members of this protein family to the secretory and mitochondrial membranes in vivo

Publisher: Federation of European Biochemical Societies. Published by Elsevier B.V.
Year: 1999
DOI identifier: 10.1016/S0014-5793(99)00581-5
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