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Discovery and functional analysis of lncRNAs: Methodologies to investigate an uncharacterized transcriptome

By Kaori Kashi, Lindsey Henderson, Alessandro Bonetti and Piero Carninci


AbstractIt is known that more than 70% of mammalian genomes are transcribed, yet the vast majority of transcripts do not code for proteins. Are these noncoding transcripts merely transcriptional noise, or do they serve a biological purpose? Recent developments in genomic analysis technologies, especially sequencing methods, have allowed researchers to create a large atlas of transcriptomes, study subcellular localization, and investigate potential interactions with proteins for a growing number of transcripts. Here, we review the current methodologies available for discovering and investigating functions of long noncoding RNAs (lncRNAs), which require a wide variety of applications to study their potential biological roles. This article is part of a Special Issue entitled: Clues to long noncoding RNA taxonomy1, edited by Dr. Tetsuro Hirose and Dr. Shinichi Nakagawa

Publisher: The Authors. Published by Elsevier B.V.
Year: 2016
DOI identifier: 10.1016/j.bbagrm.2015.10.010
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