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Redox control of hydrogenase activity in the green alga Scenedesmus obliquus by thioredoxin and other thiols

By Röbbe Wünschiers, Heinrich Heide, Hartmut Follmann, Horst Senger and Rüdiger Schulz


AbstractThe activity of the NiFe-hydrogenase from the green alga Scenedesmus obliquus is inhibited by both algal thioredoxins f and I+II, and by Escherichia coli thioredoxin. The strongest inhibition was observed with homologous chloroplastic thioredoxin f (I50=21 nM) and E. coli thioredoxin (I50=83 nM). For the homologous cytoplasmic thioredoxins I+II an I50 of 667 nM was determined. Glutathione shows a similar but much less pronounced inhibitory effect whereas dithiothreitol had no effect. In addition to glucose-6-phosphate dehydrogenase, NiFe-hydrogenase is only the second enzyme known to be inhibited by reduced thioredoxin

Publisher: Federation of European Biochemical Societies. Published by Elsevier B.V.
Year: 1999
DOI identifier: 10.1016/S0014-5793(99)00867-4
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