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Stress-induced down-regulation of tumor-associated NADH oxidase during apoptosis in transformed cells

By Liang-Chi Mao, Hsi-Ming Wang, You-Yu Lin, Te-Kau Chang, Yi-Hong Hsin and Pin Ju Chueh

Abstract

AbstractTumor-associated NADH oxidase (tNOX) is a growth-related protein expressed in transformed cells. tNOX knockdown using RNA interference leads to a significant reduction in HeLa cell proliferation and migration, indicating an important role for tNOX in growth regulation and the cancer phenotype. Here, we show that tNOX is down-regulated during apoptosis in HCT116 cells. Treatment with diverse stresses induced a dose- and time-dependent decrease in tNOX expression that was concurrent with apoptosis. Moreover, shRNA-mediated tNOX knockdown rendered cells susceptible to apoptosis, whereas re-expression of tNOX partially recovered cell proliferation. Our results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth

Publisher: Federation of European Biochemical Societies. Published by Elsevier B.V.
Year: 2008
DOI identifier: 10.1016/j.febslet.2008.09.008
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