Article thumbnail

Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited

By Florence Appaix, Marie-Nathalie Minatchy, Catherine Riva-Lavieille, Jose Olivares, Bruno Antonsson and Valdur A. Saks

Abstract

AbstractThis paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (γ) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes

Publisher: Elsevier Science B.V.
Year: 2000
DOI identifier: 10.1016/S0005-2728(00)00098-0
OAI identifier:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://s3-eu-west-1.amazonaws... (external link)
  • https://s3.amazonaws.com/prod-... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.