Article thumbnail

Proteomic Analysis of the Function of a Novel Cold-Regulated Multispanning Transmembrane Protein COR413-PM1 in Arabidopsis

By Chen Su, Kai Chen, Qingqian Ding, Yongying Mou, Rui Yang, Mengjie Zhao, Bo Ma, Zhaoshi Xu, Youzhi Ma, Yinghong Pan, Ming Chen and Yajun Xi

Abstract

The plasma membrane is the first subcellular organ that senses low temperature, and it includes some spanning transmembrane proteins that play important roles in cold regulation. COR413-PM1 is a novel multispanning transmembrane cold-regulated protein; however, the related functions are not clear in Arabidopsis. We found the tolerance to freezing stress of cor413-pm1 was lower than wild-type (WT). A proteomics method was used to analyze the differentially abundant proteins (DAPs) between cor413-pm1 and WT. A total of 4143 protein groups were identified and 3139 were accurately quantitated. The DAPs associated with COR413-PM1 and freezing treatment were mainly involved in the metabolism of fatty acids, sugars, and purine. Quantitative real-time PCR (qRT-PCR) confirmed the proteomic analysis results of four proteins: fatty acid biosynthesis 1 (FAB1) is involved in fatty acid metabolism and might affect the plasma membrane structure; fructokinase 3 (FRK3) and sucrose phosphate synthase A1 (SPSA1) play roles in sugar metabolism and may influence the ability of osmotic adjustment under freezing stress; and GLN phosphoribosyl pyrophosphate amidotransferase 2 (ASE2) affects freezing tolerance through purine metabolism pathways. In short, our results demonstrate that the multispanning transmembrane protein COR413-PM1 regulates plant tolerance to freezing stress by affecting the metabolism of fatty acids, sugars, and purine in Arabidopsis

Topics: freezing stress, COR413-PM1, gene function, proteomic analysis, Arabidopsis, Biology (General), QH301-705.5, Chemistry, QD1-999
Publisher: MDPI AG
Year: 2018
DOI identifier: 10.3390/ijms19092572
OAI identifier: oai:doaj.org/article:866d9adde67a49bf8cbfa6106fc2fb8e
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/article/866d9... (external link)
  • https://doaj.org/toc/1422-0067 (external link)
  • http://www.mdpi.com/1422-0067/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.