Article thumbnail

Prion pathogenesis is unaltered in a mouse strain with a permeable blood-brain barrier.

By Annika Keller, Mario Nuvolone, Irina Abakumova, Andra Chincisan, Regina Reimann, Merve Avar, Daniel Heinzer, Simone Hornemann, Josephin Wagner, Daniel Kirschenbaum, Fabian F Voigt, Caihong Zhu, Luca Regli, Fritjof Helmchen and Adriano Aguzzi

Abstract

Transmissible spongiform encephalopathies (TSEs) are caused by the prion, which consists essentially of PrPSc, an aggregated, conformationally modified form of the cellular prion protein (PrPC). Although TSEs can be experimentally transmitted by intracerebral inoculation, most instances of infection in the field occur through extracerebral routes. The epidemics of kuru and variant Creutzfeldt-Jakob disease were caused by dietary exposure to prions, and parenteral administration of prion-contaminated hormones has caused hundreds of iatrogenic TSEs. In all these instances, the development of postexposure prophylaxis relies on understanding of how prions propagate from the site of entry to the brain. While much evidence points to lymphoreticular invasion followed by retrograde transfer through peripheral nerves, prions are present in the blood and may conceivably cross the blood-brain barrier directly. Here we have addressed the role of the blood-brain barrier (BBB) in prion disease propagation using Pdgfbret/ret mice which possess a highly permeable BBB. We found that Pdgfbret/ret mice have a similar prion disease incubation time as their littermate controls regardless of the route of prion transmission. These surprising results indicate that BBB permeability is irrelevant to the initiation of prion disease, even when prions are administered parenterally

Topics: Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Publisher: Public Library of Science (PLoS)
Year: 2018
DOI identifier: 10.1371/journal.ppat.1007424
OAI identifier: oai:doaj.org/article:154e8661ae744cb885de7f2073c95e14
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1553-7366 (external link)
  • https://doaj.org/article/154e8... (external link)
  • https://doaj.org/toc/1553-7374 (external link)
  • http://europepmc.org/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.