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Image_2_Strategic Positioning of Connexin36 Gap Junctions Across Human Retinal Ganglion Cell Dendritic Arbors.tif

By Orsolya Kántor (642626), Gergely Szarka (6001427), Zsigmond Benkő (6001430), Zoltán Somogyvári (6001433), Emese Pálfi (6001436), Gábor Baksa (6001439), Gergely Rácz (5664262), Roland Nitschke (74660), Gábor Debertin (4429852) and Béla Völgyi (436404)

Abstract

<p>Connexin36 (Cx36) subunits form gap junctions (GJ) between neurons throughout the central nervous system. Such GJs of the mammalian retina serve the transmission, averaging and correlation of signals prior to conveying visual information to the brain. Retinal GJs have been exhaustively studied in various animal species, however, there is still a perplexing paucity of information regarding the presence and function of human retinal GJs. Particularly little is known about GJ formation of human retinal ganglion cells (hRGCs) due to the limited number of suitable experimental approaches. Compared to the neuronal coupling studies in animal models, where GJ permeable tracer injection is the gold standard method, the post-mortem nature of scarcely available human retinal samples leaves immunohistochemistry as a sole approach to obtain information on hRGC GJs. In this study Lucifer Yellow (LY) dye injections and Cx36 immunohistochemistry were performed in fixed short-post-mortem samples to stain hRGCs with complete dendritic arbors and locate dendritic Cx36 GJs. Subsequent neuronal reconstructions and morphometric analyses revealed that Cx36 plaques had a clear tendency to form clusters and particularly favored terminal dendritic segments.</p

Topics: Cell Biology, Neuroscience, Cellular Nervous System, Central Nervous System, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), Protein Trafficking, gap junction, electrical synapse, ganglion cell, inner plexiform layer, ganglion cell layer, human retina
Year: 2018
DOI identifier: 10.3389/fncel.2018.00409.s002
OAI identifier: oai:figshare.com:article/7374497
Provided by: FigShare
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