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HSV1 transcripts exhibit differential subcellular localisation.

By Kathleen Pheasant (6009734), Carla Sofia Möller-Levet (6009737), Juliet Jones (6009740), Daniel Depledge (4978244), Judith Breuer (195563) and Gillian Elliott (6009743)


<p>HFFF cells grown in slide chambers were infected with Wt virus at a multiplicity of 2, fixed at 2, 4, 6, 8 or 12 hours after infection, and processed for mRNA FISH with probes to the IE transcripts, ICP27 and ICP0, the E transcript TK and the late transcripts vhs and gC (all in red). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm.</p

Topics: Microbiology, Cell Biology, Molecular Biology, Ecology, Immunology, Science Policy, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, retention, Δ22-, cell transcriptome, proteins HSV 1 encodes, bind VP 16, HSV 1 infection, v irion h ost, host, mRNA, virus transcriptome, Wt infection, herpes simplex virus 1, translational shutoff, VP 22, mechanism, vhs endoribonuclease activity
Year: 2018
DOI identifier: 10.1371/journal.ppat.1007331.g006
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Provided by: FigShare
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