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Data_Sheet_1_Survival of Ovarian Cancer Patients Is Independent of the Presence of DC and T Cell Subsets in Ascites.docx

By Christina Wefers (6196997), Tjitske Duiveman-de Boer (6197000), Refika Yigit (6197003), Petra L. M. Zusterzeel (6197006), Anne M. van Altena (219493), Leon F. A. G. Massuger (205185) and I. Jolanda M. De Vries (6197009)

Abstract

<p>Ascites is a prominent feature of ovarian cancer and could serve as liquid biopsy to assess the immune status of patients. Tumor-infiltrating T lymphocytes are correlated with improved survival in ovarian cancer. To investigate whether immune cells in ascites are associated with patient outcome, we analyzed the amount of dendritic cell (DC) and T cell subsets in ascites from ovarian cancer patients diagnosed with high-grade serous cancer (HGSC). Ascites was collected from 62 HGSC patients prior to chemotherapy. Clinicopathological, histological and follow-up data from patients were collected. Ascites-derived immune cells were isolated using density-gradient centrifugation. The presence of myeloid DCs (BDCA-1<sup>+</sup>, BDCA-3<sup>+</sup>, CD16<sup>+</sup>), pDCs (CD123<sup>+</sup>BDCA-2<sup>+</sup>), and T cells (CD4<sup>+</sup>, CD8<sup>+</sup>) was analyzed using flow cytometry. Complete cytoreduction, response to primary treatment and chemosensitivity were associated with improved patient outcome. In contrast, immune cells in ascites did not significantly correlate with patient survival. However, we observed a trend toward improved outcome for patients having low percentages of CD4<sup>+</sup> T cells. Furthermore, we assessed the expression of co-stimulatory and co-inhibitory molecules on T cells and non-immune cells in 10 ascites samples. PD-1 was expressed by 30% of ascites-derived T cells and PD-L1 by 50% of non-immune cells. However, the percentage of DC and T cell subsets in ascites was not directly correlated to the survival of HGSC patients.</p

Topics: Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, ovarian cancer, ascites, immune environment, dendritic cells, T cells
Year: 2019
DOI identifier: 10.3389/fimmu.2018.03156.s001
OAI identifier: oai:figshare.com:article/7576436
Provided by: FigShare
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