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Protein quantification of extracellular vesicle preparation fractions from differential centrifugation and size exclusion chromatography purification.

By Chelsea N. Davis (6407891), Helen Phillips (3347261), John J. Tomes (6407894), Martin T. Swain (3290154), Toby J. Wilkinson (2016139), Peter M. Brophy (162777) and Russell M. Morphew (162753)

Abstract

<p>Fractions produced from either differential centrifugation or size exclusion chromatography methods (excretory-secretory protein, whole lysed extracellular vesicle, soluble extracellular vesicle protein and insoluble extracellular vesicle protein) were assayed for protein levels and statistically analysed (Kruskal-Wallis test, with Dunn’s Post-hoc test using Sidak correction) for differences. Asterisks identify significance where p < 0.05.</p

Topics: Biophysics, Biochemistry, Cell Biology, Biotechnology, Immunology, Developmental Biology, Marine Biology, Cancer, Infectious Diseases, Virology, Computational Biology, Chemical Sciences not elsewhere classified, equivalent EV population profiles, extracellular vesicle purification, EV purification utilised, case study, pathogen helminth EV purification, helminth pathogens Background Robust protocols, SEC purification methods, DC approaches, SEC EV preparations, EV purity, Proteomic analysis, SEC EVs, extracellular vesicles, protein identifications, cathepsin L proteases, culture media, EV purification, DC approach, purification pipeline, Protein quantification, helminth pathogens, size exclusion chromatography, purification method, ES proteins, Fasciola hepatica, EV isolation, peptide hits, application development, DC contamination, SEC methods, SEC purification realised, DC preparations, force microscopy, excretory-secretory protein, ESP, excretory-secretory products, DC EVs, tegumental artefacts
Year: 2019
DOI identifier: 10.1371/journal.pntd.0007191.g002
OAI identifier: oai:figshare.com:article/7780487
Provided by: FigShare
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