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Relaxation kinetics of the AV-HABA complex and unlabeled biotin.

By Roberto F. Delgadillo (843498), Timothy C. Mueser (5448011), Kathia Zaleta-Rivera (1334064), Katie A. Carnes (6411725), José González-Valdez (6411728) and Lawrence J. Parkhurst (61526)

Abstract

<p>The [HABA]/[B<sub>7</sub>] is the concentration ratio of these two ligands and the relaxation rate is in s<sup>-1</sup> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0204194#pone.0204194.e012" target="_blank">Eq 3</a>). The data points were fitted to a linear regression model yielding a slope and intercept of 0.0156 ± 0.0012 and 0.161 ± 0.005, respectively, resulting in a of 5.3 ± 0.9 × 10<sup>6</sup> M<sup>-1</sup>s<sup>-1</sup> at 23°C. The additional values required for this calculation were HABA association constant to the 4<sup>th</sup> site when three HABA molecules are already bound: = 5.1 ± 0.1 × 10<sup>5</sup> M<sup>-1</sup>s<sup>-1</sup> and the HABA dissociation of saturated AV-HABA<sub>4</sub> complex: = 6.23 ± 0.11 s<sup>-1</sup>. The corresponding K<sub>D</sub> <sup>AV-HABA</sup> = 12.2 ± 0.3 × 10<sup>−6</sup> M and is in excellent agreement with the 12 × 10<sup>−6</sup> M reported by Green [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0204194#pone.0204194.ref060" target="_blank">60</a>] at pH 8.</p

Topics: Biophysics, Biochemistry, Biotechnology, Evolutionary Biology, Cancer, Infectious Diseases, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, 10 7 M, association data show, surface binding studies, reaction model, drug screening assays, streptavidin, biotin, HABA, diffusion, II, avidin
Year: 2019
DOI identifier: 10.1371/journal.pone.0204194.g006
OAI identifier: oai:figshare.com:article/7788587
Provided by: FigShare
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