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Follicular and extrafollicular tetramer+ SIV-specific CD8 T cells in relation to Foxp3+ cells during early SIV infection.

By Shengbin Li (177030), Joy M. Folkvord (207887), Katalin J. Kovacs (6495377), Reece K. Wagstaff (6495380), Gwantwa Mwakalundwa (6495383), Aaron K. Rendahl (102028), Eva G. Rakasz (49023), Elizabeth Connick (207890) and Pamela J. Skinner (49026)

Abstract

<p>(<b>A</b>) Representative lymph node section stained with Mamu-A*001:01/Gag CM9 tetramers (red), IgM (blue), and Foxp3 (green) showing a tetramer+ cell in contact with a Foxp3+ cells. Confocal images were collected with a 20X objective and the scale bar is 100 μm and 10 μm in low- and high-magnification images respectively. (<b>B</b>) Representative image a showing tetramer+ Foxp3+. (<b>C</b>) Percentages of follicular tetramer+ SIV-specific CD8 T cells that were in direct contact with Foxp3+ cells tend to be lower than extrafollicular tetramer+ SIV-specific CD8 T cells (95% CI: -13.9, 1.57; p = 0.0956). <b>(D</b>) There was no significant difference between percentages of tetramer+ SIV-specific CD8+ T cells inside and outside follicle that were Foxp3+ (95% CI: -2.1, 3.4; p = 0.5676). (<b>E</b>) Representative image showing the distribution of Foxp3+ cells in lymph node. Scale bar is 100 μm. (<b>F</b>) Frequencies of Foxp3+ cells inside follicles were 67% (95% CI: 51%, 77%) lower than outside of follicle (p = 0.0007).</p

Topics: Medicine, Microbiology, Cell Biology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Plant Biology, Virology, SIV infections, Low levels, HIV, extrafollicular areas, SIV-specific, cytolytic protein perforin, B cell follicles, GC, RNA
Year: 2019
DOI identifier: 10.1371/journal.ppat.1007311.g004
OAI identifier: oai:figshare.com:article/7877396
Provided by: FigShare
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