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Verification of DEGs using qRT-PCR.

By Zhou-Xiang Liao (6597944), Zhe Ni (6597947), Xin-Li Wei (6552590), Long Chen (315739), Jian-Yuan Li (1643446), Yan-Hua Yu (6597950), Wei Jiang (14986), Bo-Le Jiang (41393), Yong-Qiang He (41391) and Sheng Huang (573383)

Abstract

<p>(A)The line shows the differences in the expression levels resulting from RNA-seq in rice leaves infected with the GX01 T3SD and GX01 wild-type (WT) strains represented as log2(FPKMT3SD/FPKMWT). The columns show expression level differences based on qRT-PCR assay results (three biological replicates). (B) The line shows the differences in the expression levels resulting from RNA-seq and displays the fold changes of GX01 growing in planta compared to in media represented as log2(FPKM <i>in planta</i>/FPKM <i>in media</i>). The columns show expression level differences in the qRT-PCR assay results (three biological replicates). All qPCR data were processed using the 2<sup>(-ΔΔC(t))</sup> method.</p

Topics: Medicine, Microbiology, Cell Biology, Ecology, Infectious Diseases, Plant Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, T 3SD strain transcriptome, plant defence response, cell wall strength, type Xoc GX 01, virulence factor synthesis, BLS, T 3SD strain, Xanthomonas oryzae pv, gene, ATP, transcription activator-like effector, TALE, T 3SS, planta, Gram-negative bacterium Xanthomonas oryzae pv
Year: 2019
DOI identifier: 10.1371/journal.pone.0215039.g003
OAI identifier: oai:figshare.com:article/8008238
Provided by: FigShare
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