Graduation date: 1968RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor\ud Virus can be fractionated into two fractions on a sucrose density\ud gradient, one of higher molecular weight and one of low molecular\ud weight. The low molecular weight (LMW) virus RNA was found to\ud chromatograph the same as E. coli tRNA on a DEAE cellulose column\ud and to have elution properties similar to myeloblast tRNA on a MAK\ud column. A portion of the LMW virus RNA fraction was demonstrated\ud to have the ability to accept amino acids in an energy requiring,\ud aminoacyl-synthetase catalyzed reaction. The acceptance exhibited\ud toward nine amino acids tested individually varied considerably.\ud The pattern of amino acid acceptance shown with LMW virus RNA\ud did not correspond to that seen with myeloblast tRNA. Finally,\ud the LMW virus RNA fraction was shown to transfer accepted amino\ud acids to a growing peptide chain in a cell-free polysome system.\ud The LMW virus RNA fraction was, thus, characterized as transfer RNA. The presence of a transfer RNA possessing a modified pattern\ud of amino acid acceptance is of significance in terms of translational\ud control of protein synthesis
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