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Direct Fluorescent Detection of Ultratrace Lanthanide(III) Ions Complexed with Aromatic Polyaminocarboxylate Avoiding Quenching of Ligand-Centered Emission Using Capillary Zone Electrophoresis with Ternary Complexing Technique

By 齋藤 伸吾

Abstract

The ultratrace level detection and the separation of lanthanide ions Ln_<3+>) were achieved using capillary zone electrophoresis with laser induced fluorescent detection (CZE-LIF) using an aromatic polyaminocarboxylate ligand synthesized in our previous work. The ligand forms kinetically stable Ln complexes at the pre-capillary derivatizing step. It effectively avoids quenching processes of the ligand-centered fluorescence through complexation with Ln_<3+>without paramagnetic and heavy atom effects because of the distance between the chelating and the antenna moieties. During the on-capillary separation step, the mother Ln complexes competitively form ternary complexes with the auxiliary ligands, iminodiacetate and citrate, which provide different mobilities for each of the Ln_<3+> complexes. The emissively labeled Ln_<3+> complexes were efficiently separated, based on the ternary complex equilibrium. Since the carrier buffer employed was free from emissive ligands, a high signal to noise ratio was obtained. A lower detection limit of 9.1 x 10(-11) mol dm(-3) (15.6 ng dm(-3), 0.46 attomole as an amount basis) was successfully achieved typically for Lu3+ with a simple CZE mode. We propose a combination of a pre-capillary and an on-capillary complexing technique as a method that provides both high sensitivity and high resolution

Publisher: Royal Society of Chemistry
Year: 2008
DOI identifier: 10.1039/b613534j
OAI identifier: oai:sucra.repo.nii.ac.jp:00013139

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