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Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis

By Ivanildes Solange da Costa Barcelos, Marcelo Simão Ferreira, Leandro Pajuaba de Moura, Germano Francisco Biondi and Julia Maria Costa-Cruz

Abstract

Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated

Topics: Taenia crassiceps, neurocysticercosis, enzyme-linked immunosorbent assay, serum, cerebrospinal fluid
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Year: 2005
OAI identifier: oai:scielo:S0074-02762005000400014
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