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Spectrophotometric determination of vitamin B2 (riboflavin) in pharmaceutical formulations using flow injection analysis

By Clezio Aniceto, Larissa de Souza Canaes, Orlando Fatibello-Filho and Carla C. S. Cavalheiro


A flow injection spectrophotometric procedure exploiting merging zones is proposed for determining vitamin B2 (riboflavin) in pharmaceutical preparations. The determination is based on the red-colored complex formation between vitamin B2 and silver(I) which was measured at 520 nm. Vitamin B2 was determined in four pharmaceutical preparations in the 1.0 to 50.0 mg L-1 concentration range, with a detection limit of 0.5 mg L-1. The recovery from three samples ranged from 98.0 to 104.0 %. The analytical frequency was 42 h-1 and r.s.d. were lower than 1% for solutions containing 10.0, 30.0 and 50.0 mg L-1 vitamin B2 (n= 10). The results obtained in commercial formulations using the FIA procedure were in good agreement with those obtained by using a conventional fluorimetric procedure (r=0.9998) and also with the label values (r= 0.9997)

Topics: vitamin B2, flow injection spectrophotometry, pharmaceutical preparations
Publisher: Sociedade Brasileira de Química
Year: 2000
OAI identifier: oai:scielo:S0100-40422000000500013
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