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Senescence in the Myxomycete Physarum Polycephalum

By Russell T.M. Poulter

Abstract

Chapter 1 consists of an introduction to the Myxomycetes (acellular slime-moulds) followed by a description of the culture techniques employed in the study of the organisms. This includes the culture of the haploid amoebae, their crossing to give diploid plasmodia, the culture of plasmodia and the induction of sporulation, and the hatching of spores to give amoebae.\ud Chapter 2 consists of a description of the analysis of fusion behaviour of plasmodia. The behaviour of two plasmodia when they make contact, fusion or non-fusion, was found to be controlled by two genes, four alleles of gene f ( f1 –f4 ) being detected, and two alleles of gene n ( n1 –n2 ). Plasmodia must have the same f genotype for fusion to be possible, and the same n phenotype, ( n2 is dominant to n1 ). One exception to this rule occurs, f3 f3 homozygotes fuse with f4 f4 homozygotes. Based partly on deductions from this exceptional observation, a general model for the mode of action of the f and n genes is proposed. The analysis of fusion type was complicated by the occurence of a recessive allele, qˉ, linked to the f gene. In the homozygous state the qˉ allele results in restricted nutritional behaviour, death occurring on the usual axenic culture medium. This is the first report of linkage in Physarum polycephalum. The genes detected during the analysis of fusion behaviour were made use of in the analysis of senescence.\ud Chapter 3 reports the further analysis of an actidione resistant mutant which, it was hoped, would be of use as a marker in the study of senescence.\ud Chapter 4 reports the application of genetic markers to the study of the ploidy of plasmodia.\ud Chapter 5 begins with an introduction to the phenomenon of senescence.\ud This is followed by the first report of senescence in a Myxomycete. Three main techniques were applied to the analysis of this phenomenon;\ud a) The life-expectancy of heterokaryons formed by fusing equal quantities of plasmodia of known, and disparate, life-expectancy was studied.\ud b) The f gene was used to study the stability of the nuclear ratios of such heterokaryons.\ud c) Various procedures, for example mutagenesis, were applied to plasmodia as a pulse, and the life-expectancy of the treated plasmodia observed to see whether senescence had been affected by the procedure.\ud The data produced by these three techniques suggests the hypothesis that senescence is due to the accumulation of defective mitochondria

Publisher: University of Leicester
Year: 1969
OAI identifier: oai:lra.le.ac.uk:2381/9814

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Citations

  1. (1969). A comparative study of the mitochondrial structure of petite strains of Saccharomaces cerevisiae. doi
  2. (1952). A comparison between the cytoplasmic components in Ph sarum polycephalum and Chaos chaos.
  3. (1967). A cytological and physiological study of synchronous mitosis in the slime-mould Physarum polycephalum.
  4. (1966). A further study of misreading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. doi
  5. (1960). A mating-type system in an acellular slime-mould. doi
  6. (1964). A protein dinier hypothesis of incompatibility.
  7. (1961). A study of plasmodium formation by the true slimemould Didymium nigripes. doi
  8. (1934). A study of the effects of modifications of the culture medium upon length of life and fecundity in a rotifer, Proales sordida, with special relation to their heritability. doi
  9. (1959). A theory of ageing. doi
  10. (1947). A transmissible, cumulative, and reversible factor in ageing. doi
  11. (1969). A yellow variant of the Eumycetozoan Didymium nigripes which exhibits ageing.
  12. (1962). Acetylcholine esterase in the plasmodium of the myxomycete Physarum polycephalum. doi
  13. (1959). Age and polyploidy of rat liver nuclei as measured by volume and DNA content. doi
  14. (1952). Age differences in respiration of guinea pig tissues. doi
  15. (1964). Age with a Future. Copenhagen: Munksgaard. xiv
  16. (1957). Ageing in the P. aurelia multimicronucleatum complex.
  17. (1966). Alterations of a maternally inherited mitochondrhl structural protein in respiratory-deficient strains of Neurosnora. doi
  18. (1952). An actomyosin-like substance from the plasmodium of a myxomycete. doi
  19. (1967). An apparent relationship between mistranslation and an altered leucyl-tRNA synthetase in a conditional lethal mutant of Neurospora crassa.
  20. (1959). An electron microscope study of flagellation in myxomycete swarm cells.
  21. (1951). An unsolved problem of biology.
  22. (1968). Antibiotic inhibitors of the bacterial ribosome.
  23. (1969). Biogenesis of mitochondria. XI. A comparison of the effects of growth limiting oxygen tenstion, intercalating agents and antibiotics in the obligate aerobe Candida parapsilosis. doi
  24. (1965). Bnzymic activities of lipofusion age pigments: comparative histochemic-al and biochemical vii studies.
  25. (1966). Chromosome numbers in pure and gross cultures of myxomycetes. doi
  26. (1956). Comparative cytochrome oxidase and atypical ascorbic acid oxidase activities in the plasmodia and spores of Physarum polycephalum.
  27. (1950). Complete development of a mycetozoon from a single spore, a single myxamoeba.
  28. (1963). Constitution of the cross-linkages in elastin. Nature, doi
  29. (1911). Die Fluoreszenz tienscher Gewebe in ultravioletten Licht. Pflugers Arch. doi
  30. (1964). Differentiations of the ground cytoplasm and the generation of the motive force for amoeboid movement.
  31. (1952). Distribution of some enzymes in the cytoplasm of the myxomycete Phy_ polycephalum.
  32. (1966). DNA synthesis by isolated nuclei of Physarum polycephalum. doi
  33. (1961). Echinostelium minutum. doi
  34. (1969). Errors in protein synthesis and clonal senescence in Fungi. doi
  35. (1956). Experiential 4j 19.
  36. (1960). Flagella formation by myxamoebae of the true slime-mould Didymium nigripes. doi
  37. (1957). Genesis of intracellular pigment on the spinal ganglia of senile rats. An electron microscope study. doi
  38. (1960). Genetic analysis of a myxomycete. doi
  39. (1966). Genetic analysis of actidione-resistant mutants in the Myxomycete, Physarum polycephalum, doi
  40. (1969). Genetics and phenogenetics of mitochondria. doi
  41. (1968). Genetics of plasmodial compatibility and heterokaryosis in Didymium iridis. doi
  42. (1967). Hormonal Effects in Relation to Ageing in Mammals.
  43. (1960). Human hepatocellular pigments and lysosomes. doi
  44. (1965). Identification of a new crosslinking amino acid in elastin. doi
  45. (1956). IIber dem Nachweis von hydrolytischen enzymen in
  46. (1969). Induction of cytoplasmic respiratory deficiency in yeast by phenethylalcohol. doi
  47. (1968). Inheritance of the "life-spanning" phenomenon in Amoeba rop teus. doi
  48. (1966). Interference of 5-fluorouracil in the bio-synthesis of ribosomes in Staphyloccocus aureus (strain Duncan). doi
  49. (1967). Invertebrates, immunology and evolution.
  50. (1964). Irreversible cellular changes in old mice. doi
  51. (1954). Lack of an effect of a high dose of X rays on ageing
  52. (1959). Lethal suppressive cytopla. 3 in aged clones of Asperig llus glaucus.
  53. (1966). Light induced synchronous sporulation of a myxomycete. doi
  54. (1966). Limited DNA synthesis in the absence of protein synthesis in Physarum polycephalum. doi
  55. (1955). Meiosis in the myxouycetes.
  56. (1964). Methods of culture for plasmodiaiMyxomycetes.
  57. (1952). Mineral requirements of some myxomycetes with possible bearing on their ecology.
  58. (1956). Mitochondrial changes in different physiological states. doi
  59. (1964). Mitotic synchrony in the plasmodia of Ph Sarum polycephalum and mitotic synchronisation by coalescence of microplasmodia. doi
  60. (1963). Modes of intermolecular cross-linking in mature insoluble collagen.
  61. (1967). Modified ribosomes conferring resistance to cyclohexamide in mutants of Saccharomyces cerevisiae.
  62. (1967). Molecular Aspects of Ageing.
  63. (1961). Morphogenesis of the sporagium of Comatricha. doi
  64. (1961). Morphological observations on growth and differentiation of Physarum polycephalum grown in pure culture. doi
  65. (1960). Nucleic acid metabolism in a slime mould with synchronous mitosis. doi
  66. (1959). On the nature of the ageing process. doi
  67. (1965). Optimal conditions for mutagenesis by NMG in E. coli K12. doi
  68. (1944). Paramecium bursaria: Life history: III. Repeated conjugations in the same stock 'a. t different ages, with and without inbreeding, in relation to mortality at conjugation. doi
  69. (1965). Periodische Anderungen der Thymidinkinase Aktivitat in Synchronen Mitosecyclus von Physarum polycephalum.
  70. (1968). Phenethyl alcohol: reversible inhibition of synthesis of macromolecules and disaggregation of polysomes in rat hepatoma cells. doi
  71. (1966). Plasmodial compatibility in heterothallic and homothallic isolates of Didymium iridis. doi
  72. (1967). Plasmodial fusion and lethal interaction between strains in a myxomycete. doi
  73. (1964). Plastids and mitochondria: Inheritable systems. doi
  74. (1959). Protoplasmic streaming. doi
  75. (1962). Purification and some properties of a c-type cytochrome from a slime-mould Ph sarum polycephalum. doi
  76. (1939). Races physiologiques chez les myxomycetes Didymium
  77. (1957). Radiation induced presumed somatic mutations in the house mouse.
  78. (1962). Recombination in a Myxomycete. doi
  79. (1966). Regulation of chromosome replication in E. coli: A comparison of the effects of phemethyl alcohol treatment with those of amino acid starvation. doi
  80. (1966). Ribonucleic acid synthesis in vivo in the synchronously dividing Physarum polycephalum. doi
  81. (1964). RNA synthesis in the mitotic cycle of Physarum polycephalum. doi
  82. (1967). Senescence and the fidelity of protein synthesis in Drosophila. doi
  83. (1961). Senescence and the metabolism of various tissues of rats.
  84. (1965). Sequential temporal replication of DNA in Physarum polycephalum. doi
  85. (1948). Structure and biogenesis of emetine. doi
  86. (1961). Studies of human and tunicate cellulose and of their relations to reticulin. doi
  87. (1964). Studies on the life cycle of Ph sarum rosum and other myxomycetes. Mycologia, 56s 170. doi
  88. (1964). Studies on the ribosomes of streptomycinsensitive and resistant strains of Escherichia coli. doi
  89. (1953). Sur 1'impossibilitd dtobtenir la multiplication veggtative ininterrompue et illimite de l'Ascomycete Podospora anserina.
  90. (1937). Sur l'existence des races physiologiques chez les myxomycetes.
  91. (1927). Sur la fusion des plasmodium des myxomycetes.
  92. (1967). Symposia XXI
  93. (1956). Synergism of two cytoplasmically inherited mutants in Neuros ora crassa, doi
  94. (1957). Syngamy and plasmodium formation in the Myxoccatres" doi
  95. (1963). The accumulation of free fatty acids in poky, a maternally inherited mutant of N©urospora crassa. doi
  96. (1967). The ageing of connective tissue. doi
  97. (1968). The Biogenesis of Mitochondria. doi
  98. (1960). The Biology of Ageing,
  99. (1952). The case of "maternal" inheritance in Neurospora crassa. doi
  100. (1962). The causes of ageing.
  101. (1968). The development of flagella in swarm cells of a myxomycete Physarum flavicomum. doi
  102. (1967). The effect of ageing upon collagen catabolism.
  103. (1938). The effect of light on the fruiting of Myxomycetes. doi
  104. (1945). The existence of physiological strains of Physarum polycephalum. doi
  105. (1960). The experimental production of senile pigments in the nerve cells of young rats. doi
  106. (1955). The fine structure of young and old spinal ganglia. doi
  107. (1951). The gene that causes natural death
  108. (1965). The general occurrence of mitochondrial DNA. doi
  109. (1955). The genetics and cytology of Drosophila subobscura. doi
  110. (1939). The Golgi apparatus in the nerve cells of the mouse from youth to senility. doi
  111. (1965). The limited in vitro lifetime of human diploid cell strains. doi
  112. (1963). The maintenance of the accuracy of protein synthesis and its relevance to ageing. doi
  113. (1961). The modification by X-rays of the life span of haploids and diploids of the wasp, Habrobracan species. doi
  114. (1967). The nature of cellular age changes.
  115. (1907). The problem of age, growth and death. Popular Sci., doi
  116. (1950). The relation of cholinesterase activity to change in cell number with age in the brain of the adult worker honey bee. doi
  117. (1967). The role of DNA lesions in the processes leading to ageing in mice.
  118. (1949). The swarm cells of Myxomycetes. doi
  119. (1961). The systematic position of the myxomycetes. doi
  120. (1967). The ultrastructure of meiosis in three species of Physarum. Mycologia, doi
  121. (1962). Time, Cells and Ageing, doi
  122. (1967). Transplantation of nuclei and mitochondria of Physarum polycephalurn by plasmodia coalescence. doi
  123. (1961). Turnover of rat liver mitochondria. doi
  124. Unpublished data quoted

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