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Synthesis of the flavonoid 3′,4′,5′-trimethoxyflavonol and its determination in plasma and tissues of mice by HPLC with fluorescence detection

By Robert G. Britton, Isabel Fong, Shaban Saad, Karen Brown, William P. Steward, Andreas J. Gescher and Stewart Sale

Abstract

This paper was published as Journal of Chromatography B, 2009, 877 (10), pp. 939-942. It is available from http://www.sciencedirect.com/science/article/pii/S1570023209001275. DOI: 10.1016/j.jchromb.2009.02.043Metadata only entry3′,4′,5′-Trimethoxyflavonol (TMFol) was synthesized as a potential colorectal cancer chemopreventive agent. An HPLC method for determination for TMFol in murine plasma and tissues was developed and validated using human plasma. Analyte was separated (C18 column; fluorescence detection 330 nm excitation, 440 nm emission) using 69% methanol and 0.1 M ammonium acetate buffer (pH 5.1) as mobile phase. The method was linear for 50–2500 ng/ml plasma and 0.05–10 μg/g tissue (r > 0.99). TMFol was recovered from plasma or tissues using solid phase columns or organic solvent protein precipitation, respectively. Recovery at low, medium and high concentrations was 97.6–107.3%, with inter- and intra-day coefficients of variation of <10%. The lower limit of quantitation for plasma was 50 ng/ml. The method was applied to measure steady-state TMFol plasma and tissue levels in mice which received dietary TMFol (0.2%)

Topics: 3′,4′,5′-Trimethoxyflavonol, High performance liquid chromatography, Cancer chemoprevention
Publisher: Elsevier
Year: 2009
DOI identifier: 10.1016/j.jchromb.2009.02.043
OAI identifier: oai:lra.le.ac.uk:2381/9491
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