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Anthocyanin-rich red grape extract impedes adenoma development in the ApcMin mouse: Pharmacodynamic changes and anthocyanin levels in the murine biophase

By Hong Cai, Timothy H. Marczylo, Nicole Teller, Karen Brown, William P. Steward, Doris Marko and Andreas J. Gescher

Abstract

This paper was published as European Journal of Cancer, 2010, 46 (4), pp. 811-817. It is available from http://www.sciencedirect.com/science/journal/09598049. DOI: 10.1016/j.ejca.2009.12.017Metadata only entryPurpose:\ud Red grape pomace extract (oenocyanin) is a cheap and rich source of anthocyanins, the agents suggested to possess cancer chemopreventive properties. Here the hypothesis was tested that oenocyanin added to the diet can interfere with intestinal adenoma development in the ApcMin mouse, a model of intestinal carcinogenesis linked to an Apc mutation.\ud Methods:\ud Mice received oenocyanin (0.3%) in their diet until week 16, when adenoma number and burden were recorded. Expression of Akt and ERK proteins was studied by Western blot in adenomas to discover effects of anthocyanins on cellular signalling via the PI3 and MAP kinase pathways. Levels of anthocyanins were measured by HPLC with visible spectroscopic or mass spectrometric detection.\ud Results:\ud In mice which had consumed oenocyanin, overall adenoma burden was halved and adenoma number was marginally reduced when compared with mice on control diet. The proliferation index in colonic adenomatous crypts, as reflected by Ki-67 staining, was significantly decreased from 88.14% in control mice to 75.6 ± 4% in mice on oenocyanin (P = 0.014). Expression of Akt in small intestinal adenomas from ApcMin mice on oenocyanin was reduced by 54% (P = 0.003), when compared to controls. Oenocyanin anthocyanins and glucuronide metabolites were found in the urine and intestine but not in plasma.\ud Conclusions:\ud The results suggest that oenocyanin may be a viable and economical alternative to anthocyanin-rich berry extracts for chemopreventive intervention. Akt and pErk might be suitable biomarkers of anthocyanin target organ efficacy

Topics: Anthocyanins, Chemoprevention, Drug development
Publisher: Elsevier
Year: 2010
DOI identifier: 10.1016/j.ejca.2009.12.017
OAI identifier: oai:lra.le.ac.uk:2381/9484
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