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Fluorescent in situ hybridization and flow cytometry\ud as tools to evaluate the treatments for the control\ud of slime-forming enterobacteria in paper mills

By Claudia Esperanza Torres García, Alicia Gibello, Mar Nande, Margarita Martín and Ángeles Blanco Suárez

Abstract

Slime formation is a serious problem nowadays\ud in the paper industry. Some enterobacteria are associated\ud with the formation of slime deposits in paper and board\ud mills. Detection and characterization of slime forming\ud bacteria, belonging to the genus Enterobacter, Raoultella,\ud and Klebsiella have been achieved by fluorescence in situ\ud hybridization (FISH), using one probe based on the\ud enterobacterial repetitive intergenic consensus sequence\ud and other two rRNA targeted oligonucleotide probes. The\ud effects of three kinds of antimicrobiological products\ud (biocides, dispersants, and enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming enterobacteria and to select specific treatments to control microbial problems in the paper industry

Topics: Agua, Biología molecular, Ingeniería química, Bioquímica
Publisher: Springer
Year: 2008
DOI identifier: 10.1007/s00253-008-1369-6
OAI identifier: oai:www.ucm.es:11899
Provided by: EPrints Complutense

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