Skip to main content
Article thumbnail
Location of Repository

Epistatic interactions of spontaneous mutations in haploid strains of the yeast Saccharomyces cerevisiae

By Dominika M Wloch, Rhona H. Borts and Ryszard Korona


The full text of this article is not available on the LRA. The published version can be found at\ud DOI: 10.1046/j.1420-9101.2001.00267.xSeveral important biological phenomena, including genetic recombination\ud and sexual reproduction, could have evolved to counteract genome contamination\ud by deleterious mutations. This postulate would be especially relevant if\ud it were shown that deleterious mutations interact in such a way that their\ud individual negative effects are reinforced by each other. The hypothesis of\ud synergism can be tested experimentally by crossing organisms bearing\ud deleterious mutations and comparing the ®tness of the parents and their\ud progeny. The present study used laboratory strains of the budding yeast\ud burdened with mutations resulting from absence of a major DNA mismatch\ud repair function. Only in one, or possibly two, crosses out of eight did ®tness of\ud the progeny deviate from that of their parents in a direction indicating\ud synergism. Furthermore, the distributions of progeny ®tness were not skewed\ud as would be expected if strong interactions were present. The choice of\ud experimental material ensured that genetic recombination was extensive, all\ud four meiotic products were available for ®tness assays, and that the mutations\ud were probably numerous. Despite this generally favourable experimental\ud setting, synergism did not appear to be a dominating force shaping ®tness of\ud yeast containing randomly generated mutations

Topics: epistasis, fitness, Saccharomyces cerevisiae, spontaneous mutation
Publisher: Wiley-Blackwell
Year: 2001
DOI identifier: 10.1046/j.1420-9101.2001.00267.x
OAI identifier:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • (external link)
  • http://www.blackwellpublishing... (external link)
  • http://www.blackwellpublishing... (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.