Location of Repository

Temperature-Dependent Modulation of Chromosome Segregation in msh4 Mutants of Budding Yeast

By Andrew Chi-Ho Chan, Rhona H. Borts and Eva R. Hoffmann


Background: In many organisms, homologous chromosomes rely upon recombination-mediated linkages, termed\ud crossovers, to promote their accurate segregation at meiosis I. In budding yeast, the evolutionarily conserved mismatchrepair\ud paralogues, Msh4 and Msh5, promote crossover formation in conjunction with several other proteins, collectively\ud termed the Synapsis Initiation Complex (SIC) proteins or ‘ZMM’s (Zip1-Zip2-Zip3-Zip4-Spo16, Msh4-Msh5, Mer3). zmm\ud mutants show decreased levels of crossovers and increased chromosome missegregation, which is thought to cause\ud decreased spore viability.\ud Principal Findings: In contrast to other ZMM mutants, msh4 and msh5 mutants show improved spore viability and\ud chromosome segregation in response to elevated temperature (23°C versus 33°C). Crossover frequencies in the population\ud of viable spores in msh4 and msh5 mutants are similar at both temperatures, suggesting that temperature-mediated\ud chromosome segregation does not occur by increasing crossover frequencies. Furthermore, meiotic progression defects at\ud elevated temperature do not select for a subpopulation of cells with improved segregation. Instead, another ZMM protein,\ud Zip1, is important for the temperature-dependent improvement in spore viability.\ud Conclusions: Our data demonstrate interactions between genetic (zmm status) and environmental factors in determining\ud chromosome segregation

Publisher: Public Library of Science
Year: 2009
DOI identifier: 10.1371/journal.pone.0007284
OAI identifier: oai:lra.le.ac.uk:2381/8864

Suggested articles



  1. (2004). A role for centromere pairing in meiotic chromosome segregation.
  2. (2007). Amon A doi
  3. (1986). An alternative pathway for meiotic chromosome segregation in yeast. doi
  4. (1949). Biochemical mutants in the smut fungus Ustilago maydis. doi
  5. (2007). BLM ortholog, Sgs1, prevents aberrant crossing-over by suppressing formation of multichromatid joint molecules. doi
  6. (2004). Competing crossover pathways act during meiosis in Saccharomyces cerevisiae. doi
  7. (1997). Conserved properties between functionally distinct MutS homologs in yeast. doi
  8. (2006). Crossover homeostasis in yeast meiosis. doi
  9. (2004). Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis. doi
  10. (2000). Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA.
  11. (2001). Differential timing and control of noncrossover and crossover recombination during meiosis. doi
  12. (2008). Global analysis of the meiotic crossover landscape. doi
  13. (2008). Highresolution mapping of meiotic crossovers and non-crossovers in yeast. doi
  14. (2004). hMSH4-hMSH5 recognizes Holliday Junctions and forms a meiosis-specific sliding clamp that embraces homologous chromosomes. doi
  15. (1994). How meiotic cells deal with non-exchange chromosomes. doi
  16. (1995). Identification of double Holliday junctions as intermediates in meiotic recombination. doi
  17. (1994). Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis. doi
  18. (1989). Lack of DNA homology in a pair of divergent chromosomes greatly sensitizes them to loss by DNA damage. doi
  19. (2006). Meiotic chromosome synapsis-promoting proteins antagonize the anti-crossover activity of Sgs1. doi
  20. (2004). Meiotic recombination intermediates and mismatch repair proteins. doi
  21. (1995). MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repair. doi
  22. (2005). Multiple branches of the meiotic recombination pathway contribute independently to homolog pairing and stable juxtaposition during meiosis in budding yeast. doi
  23. (1994). Mutation of a meiosis-specific MutS homolog decreases crossing-over but not mismatch correction. doi
  24. (1994). Physical association between nonhomologous chromosomes precedes distributive disjunction in yeast. doi
  25. (2009). Population genomics of domestic and wild yeasts. doi
  26. (2003). Sequencing and comparison of yeast species to identify genes and regulatory elements. doi
  27. (2001). The budding yeast Msh4 protein functions in chromosome synapsis and the regulation of crossover distribution.
  28. (2000). The crystal structure of DNA mismatch repair protein MutS binding to a G*T mismatch. doi
  29. (1996). The mismatch repair system reduces meiotic homeologous recombination and stimulates recombination-dependent chromosome loss. doi
  30. (2005). The roles of MAD1, MAD2 and MAD3 in meiotic progression and the segregation of nonexchange chromosomes. doi
  31. (2001). The single-end invasion: an asymmetric intermediate at the double- strand break to double-holliday junction transition of meiotic recombination.
  32. (2007). The spindle checkpoint rescues the meiotic segregation of chromosomes whose crossovers are far from the centromere. doi
  33. (2003). Un menage a quatre: the molecular biology of chromosome segregation in meiosis. doi
  34. (1998). Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.