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Temperature-Dependent Modulation of Chromosome Segregation in msh4 Mutants of Budding Yeast

By Andrew Chi-Ho Chan, Rhona H. Borts and Eva R. Hoffmann

Abstract

Background: In many organisms, homologous chromosomes rely upon recombination-mediated linkages, termed\ud crossovers, to promote their accurate segregation at meiosis I. In budding yeast, the evolutionarily conserved mismatchrepair\ud paralogues, Msh4 and Msh5, promote crossover formation in conjunction with several other proteins, collectively\ud termed the Synapsis Initiation Complex (SIC) proteins or ‘ZMM’s (Zip1-Zip2-Zip3-Zip4-Spo16, Msh4-Msh5, Mer3). zmm\ud mutants show decreased levels of crossovers and increased chromosome missegregation, which is thought to cause\ud decreased spore viability.\ud Principal Findings: In contrast to other ZMM mutants, msh4 and msh5 mutants show improved spore viability and\ud chromosome segregation in response to elevated temperature (23°C versus 33°C). Crossover frequencies in the population\ud of viable spores in msh4 and msh5 mutants are similar at both temperatures, suggesting that temperature-mediated\ud chromosome segregation does not occur by increasing crossover frequencies. Furthermore, meiotic progression defects at\ud elevated temperature do not select for a subpopulation of cells with improved segregation. Instead, another ZMM protein,\ud Zip1, is important for the temperature-dependent improvement in spore viability.\ud Conclusions: Our data demonstrate interactions between genetic (zmm status) and environmental factors in determining\ud chromosome segregation

Publisher: Public Library of Science
Year: 2009
DOI identifier: 10.1371/journal.pone.0007284
OAI identifier: oai:lra.le.ac.uk:2381/8864

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