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Genomic 3D compartments emerge from unfolding mitotic chromosomes

By Rajendra Kumar, Ludvig Lizana and Per Stenberg

Abstract

The 3D organisation of the genome in interphase cells is not a randomly folded polymer. Rather, experiments show that chromosomes arrange into a network of 3D compartments that correlate with biological processes, such as transcription, chromatin modifications and protein binding. However, these compartments do not exist during cell division when the DNA is condensed, and it is unclear how and when they emerge. In this paper, we focus on the early stages after cell division as the chromosomes start to decondense. We use a simple polymer model to understand the types of 3D structures that emerge from local unfolding of a compact initial state. From simulations, we recover 3D compartments, such as TADs and A/B compartments that are consistently detected in chromosome capture experiments across cell types and organisms. This suggests that the large-scale 3D organisation is a result of an inflation process

Topics: Nuclear structure, Polymer simulation, Chromosome decondensation, Hi-C, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi
Publisher: 'Springer Science and Business Media LLC'
Year: 2019
DOI identifier: 10.1007/s00412-018-0684-7
OAI identifier: oai:DiVA.org:umu-157199
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