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The endogenous production of hydrogen sulphide in intrauterine tissues

By Pushpa Patel, Manu Vatish, John Heptinstall, Rui Wang and Ray J. Carson

Abstract

Background: Hydrogen sulphide is a gas signalling molecule which is produced endogenously from\ud L-cysteine via the enzymes cystathionine beta-synthase (CBS) and cystathionine gamma-lyase\ud (CSE). The possible role of hydrogen sulphide in reproduction has not yet been fully investigated.\ud It has been previously demonstrated that hydrogen sulphide relaxes uterine smooth muscle in\ud vitro. The aim of the present study was to investigate the endogenous production of hydrogen\ud sulphide in rat and human intrauterine tissues in vitro.\ud Methods: The production of hydrogen sulphide in rat and human intrauterine tissues was\ud measured in vitro using a standard technique. The expression of CBS and CSE was also investigated\ud in rat and human intrauterine tissues via Western blotting. Furthermore, the effects of nitric oxide\ud (NO) and low oxygen conditions on the production rates of hydrogen sulphide were investigated.\ud Results: The order of hydrogen sulphide production rates (mean +/- SD, n = 4) for rat tissues\ud were: liver (777 +/- 163 nM/min/g) > uterus (168 +/- 100 nM/min/g) > fetal membranes (22.3 +/-\ud 15.0 nM/min/g) > placenta (11.1 +/- 4.7 nM/min/g), compared to human placenta (200 +/- 102 nM/\ud min/g). NO significantly increased hydrogen sulphide production in rat fetal membranes (P < 0.05).\ud Under low oxygen conditions the production of hydrogen sulphide was significantly elevated in\ud human placenta, rat liver, uterus and fetal membranes (P < 0.05). Western blotting (n = 4) detected\ud the expression of CBS and CSE in all rat intrauterine tissues, and in human placenta, myometrium,\ud amnion and chorion.\ud Conclusion: Rat and human intrauterine tissues produce hydrogen sulphide in vitro possibly via\ud CBS and CSE enzymes. NO increased the production of hydrogen sulphide in rat fetal membranes.\ud The augmentation of hydrogen sulphide production in human intrauterine tissues in a low oxygen\ud environment could have a role in pathophysiology of pregnancy

Publisher: BioMed Central
Year: 2009
DOI identifier: 10.1186/1477-7827-7-10
OAI identifier: oai:lra.le.ac.uk:2381/8100
Journal:

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Citations

  1. (1998). A: Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution. doi
  2. (1998). A: Hemeoxygenase-1 inhibits human myometrial contractility via carbon monoxide and is upregulated by progesterone during pregnancy. doi
  3. (1982). Beck PW: Charcaterization of the enzymic capacity for cysteine desulphhydration in liver and kidney of the rat. doi
  4. (1984). Biosynthesis and proteolytic activation of cystathionine beta-synthase in rat liver.
  5. (2005). Caniggia I: Molecular Evidence of Placental Hypoxia in Preeeclampsia. doi
  6. (1987). Cystathionine beta-synthase (human). Methods Enzymol doi
  7. (1984). Cystathionine gamma-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine gamma-lyase in filamentous bacteria and its purification and characterization. doi
  8. (1965). Enzymatic synthesis and cleavage of cystathionine in fungi and bacteria.
  9. (1990). Glode LM: Sequence of cDNA for rat cystathionine gamma-lyase and comparison of deduced amino acid sequence with related Escherichia coli enzymes. doi
  10. (1958). Greenberg DM: A crystalline enzyme that cleaves homoserine and cystathionine I. Isolation procedure and some physiochemical properties.
  11. (2004). Hydrogen sulphideinduced relaxation of resistance mesenteric artery beds of rats.
  12. (1995). Involvement of a nitric oxide-cyclic guanosine monophosphate pathway in control of human uterine contractility during pregnancy. doi
  13. (1992). JP: Rat cystathionine beta-synthase. Gene organization and alternative splicing. doi
  14. (2002). JT: Hormonal regulation of cystathionine beta-synthase expression in liver.
  15. (1992). Kraus JP: Rat cystathionine beta-synthase: expression of four alternatively spliced isoforms in transfected cultured cells. Arch Biochem Biophys doi
  16. (2003). Modulation of endogenous production of H2S in rat tissues. doi
  17. (2005). PK: Role of hydrogen sulphide in acute pancreatitis and associated lung injury. J Fed Am Soc Exp Biol
  18. (2004). PK: Role of hydrogen sulphide in haemorrhagic shock in the rat: protective effect of inhibitors of hydrogen sulphide biosynthesis. doi
  19. (2002). PK: The smooth muscle relaxant effect of hydrogen sulphide in vitro:evidence for a physiological role to control intestinal contractility. doi
  20. (1978). Purification and properties of cystathionine β-synthase from human liver.
  21. (2001). RJ: L-cysteine and sodium hydrosulphide inhibit spontaneous contractility in isolated pregnant uterine strips in vitro. Pharmacol Toxicol doi
  22. (1998). Role and regulation of nitric oxide in uterus during pregnancy and parturition. doi
  23. (2005). Role of oxidative stress in female reproduction. Reprod Biol Endocrinol
  24. (1996). The possible role of hydrogen sulphide as an endogenous neuromodulator.
  25. (1997). The possible role of hydrogen sulphide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem Biophys Res Comm doi
  26. (1942). The utilization of cysteine and cystine by rat liver with the production of hydrogen sulphide.
  27. (2001). The vasorelaxant effect of HS as a novel endogenous gaseous KATP channel opener. doi
  28. (2005). Thiemermann C: Inhibition of endogenous hydrogen sulphide formation reduces the organ injury caused by endotoxemia. doi
  29. (2002). Two's company, three's a crowd – can H2S be the third endogenous gaseous transmitter? J Fed Am Soc Expl Biol doi
  30. (2002). Vitamin E and C supplementation in women at risk of pre-eclampsia is associated with changes in indices of oxidative stress and placental function. doi
  31. (2000). Wargovich MJ: Direct reprobing with anti-b-actin as an internal control for western blot analysis. Biotechniques

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