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Metagenomic Accessing of Genes From Environmental Clone Library From an Oxidation Tank of a Waste Treatment Plant Using 59-be Conserved Sequence Specific Primers

By Muhammad Afzal Ghauri, William Duncan Grant and Shaun Heaphy


This paper was published as Pakistan Journal of Zoology, 2010, 42 (1), pp. 33-36. It is available from only entryEnvironmental samples were collected from oxidation tank of a waste treatment plant in Leicester, United Kingdom. Genomic DNA was recovered from the environmental samples and amplified by PCR using 59-be conserved sequence specific primers HS286 and HS287. Different 59-be conserved sequence containing genes were recovered from the environmental DNA clone library. Most of the recovered genes were novel with no identifiable ORF homologues in the databases. This technique demonstrates the usefulness of a culture-independent gene mining approach as an alternative to traditional gene recovery technologies

Publisher: Zoological Society of Pakistan
Year: 2010
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